Immunological and Morphological Assessment of Diagnostic Agglutinating Sera for Brucellosis

Brucellosis, Brucella abortus S19, diagnostic agglutinating antiserum, hyperimmunization, immunoglobulins, immunological evaluation, morphological evaluation, rabbit, serological diagnosis

1*Yusupov Akmal Pulatovich
Alfraganus University, Department of Medicine,
PhD (Doctor of Philosophy),
Associate Professor, Tashkent, Uzbekistan.
E-mail: a.yusupov@afu.uz
ORCID:https://orcid.org/0009-0007-4167-5433

2Tuychiev Laziz Nadirovich
a Department of Infectious and Children's Infectious
Diseases, MD, Professor Tashkent State Medical
University, Tashkent, Uzbekistan.
b Research Institute of the Republican Specialized
Scientific and Practical Medical Center of
Epidemiology, Microbiology, Infectious and
Parasitic Diseases, Tashkent, Uzbekistan.
E-mail: l_tuychiev@mail.ru
ORCID:https://orcid.org/0000-0003-2312-8640

3Khudaykulova Gulnara
Head of the Department of Public Health &
Healthcare Management, DSc, Professor. Tashkent
State Medical University, Tashkent, Uzbekistan.
E-mail: gkhudaykulova@gmail.com
ORCID:https://orcid.org/0009-0003-2238-7373

4Rakhmatullaeva Shakhnoza Bakhadirovna
Professor, Department of Infectious and Children's
Infectious Diseases, Doctor of Medical Sciences
(DSc), Tashkent State Medical University,
Tashkent, Uzbekistan.
E-mail: Doctor_shakhnoza@mail.ru
ORCID:https://orcid.org/0000-0001-7257-2081

5Muminova Makhbuba Teshayevna
Department of Infectious and Children's Infectious
Diseases, PhD, associate professor
Tashkent State Medical University, Tashkent,
Uzbekistan.
E-mail: maxbubamuminova515@gmail.com
ORCID:https://orcid.org/0000-0001-8194-8052

6Akhmedova Oydin Anvarovna
Assistant, Department of Infectious and Children's
Infectious Diseases, Tashkent State Medical
University, Tashkent, Uzbekistan.
E-mail: akhmedovaoydin80@gmail.com
ORCID:https://orcid.org/0009-0005-8635-1454

7Ikramov Umirzoq Eminjonovich
Assistant, Department of Infectious and Children's
Infectious Diseases, Tashkent State Medical
University, Tashkent, Uzbekistan.
E-mail: umrzoqikromov1994@gmail.com
ORCID:https://orcid.org/0009-0000-8336-1888

8Saidova Sitora
Assistant, Department of Infectious and Children's
Infectious Diseases, Tashkent State Medical
University, Tashkent, Uzbekistan.
E-mail: s.saidova@tashmeduni.uz
ORCID:https://orcid.org/0009-0007-4882-2854

Received-18-05-2026
Revised-22-06-2026
Accepted-27-06-2026

European Journal of Prosthodontics and Restorative Dentistry (2026) 34 (4s), 569-577

Immunological and Morphological Assessment of Diagnostic Agglutinating Sera for Brucellosis

The aim of this study was to obtain hyperimmune diagnostic agglutinating antiserum against brucellosis by hyperimmunizing experimental rabbits with the live vaccine strain Brucella abortus 19 (BA19) in combination with incomplete Freund’s adjuvant, and to evaluate the morphological changes occurring in the liver, spleen, and kidneys during the immunization process. The production of specific antibodies in the obtained antisera was assessed using serological methods, while morphological alterations in the internal organs were investigated by macroscopic and histological examinations. The results demonstrated that hyperimmunization induced the production of high-titer diagnostic hyperimmune antiserum in rabbits. In addition, active immunogenesis was observed in the spleen, whereas predominantly reversible morphological changes developed in the liver and kidneys. These findings provide a scientific basis for the development of high-quality diagnostic agglutinating antisera for the serological diagnosis of brucellosis and contribute to a better understanding of their associated morphological characteristics.

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