Immunomodulatory Effects of Multi-Component Dietary Supplementation on Humoral and Cellular Immune Responses: A Randomized Controlled Trial

Immunomodulation; tissue hydrolysate; ascorbic acid; randomized controlled trial; NK cells.

Atamukxamedova Dilafruz1
Department of infectious diseases and pediatric
infectious diseases, phthisiology and
pulmonology, Tashkent State Medical
University, Tashkent, Uzbekistan.
E-mail: dilafruzatamuxamedova@gmail.com
https://orcid.org/0000-0002-3981-0081

Sadullaev Siroj*2
Department of Infectious Diseases,
Epidemiology and Phthisiology,
Urgench State Medical Institute, Urgench,
Uzbekistan.
E-mail: sadullayev.siroje@gmail.com
https://orcid.org/0009-0006-4052-7183

Akhrarova Azizakhon3
Department of Internal Diseases, Alfraganus
University, Tashkent, Uzbekistan.
E-mail: azizakhonakhrarova@gmail.com
Orcid: https://orcid.org/0009-0007-4169-4116

Muminova Nilufar4
Department of Internal Diseases, Alfraganus
University, Tashkent, Uzbekistan.
E-mail: muminovanilufarxon1@gmail.com
Orcid: https://orcid.org/0009-0002-3353-0406

Mamanzarova Nazokat5
Resident at Tashkent State Medical University,
Tashkent, Uzbekistan.
email: nazokattolipova530@gmail.com
https://orcid.org/0009-0003-7038-3836

Matkarimov Matkarim6
Student Faculty of 2-General Medicine,
Urgench State Medical Institute, Urgench,
Uzbekistan.
E-mail: matkarimmatkarimov0804@gmail.com
https://orcid.org/0009-0001-8598-9924

Samadov Bakhodirjon7
a Department of Pharmacology, Bukhara State
Medical Institute named after Abu Ali ibn Sino,
Bukhara, Uzbekistan;
E-mail: samadov.baxodirjon@bsmi.uz

b Department of Clinical Sciences, Asia
International University, Bukhara, Uzbekistan
Email: samadov.baxodirjon@oxu.uz
c Research fellow at the University of Tashkent
for Applied Sciences, 1, Gavkhar street,
Chilanzar district, Tashkent, Uzbekistan;
E-mail: baxodir.samadov48@gmail.com
Orcid: https://orcid.org/0000-0003-4593-6569

Received-20-05-2026
Revised-24-06-2026
Accepted-27-06-20.26

European Journal of Prosthodontics and Restorative Dentistry (2026) 34 (4s), 531-539

Immunomodulatory Effects of Multi-Component Dietary Supplementation on Humoral and Cellular Immune Responses: A Randomized Controlled Trial

Multi-component dietary supplements targeting immune function represent a growing clinical intervention strategy. This randomized, double-blind, placebo-controlled trial evaluated the immunomodulatory effects of IMMUN-5 — a capsule-based supplement containing tissue hydrolysate (1 mg), ascorbic acid (25 mg), and menthol (5 mg) — in 120 adults with immunodeficiency-associated conditions over 60 days. Participants received IMMUN-5 (two capsules twice daily) or identical placebo. Outcomes included serum IgG, IgM, and IgA concentrations, CD3+/CD4+/CD8+ T-lymphocyte percentages, NK cell frequency and cytotoxicity, and phagocytic index, assessed at baseline, Week 4, and Week 8. The IMMUN-5 group demonstrated significantly greater increases in IgG (+34.7%), IgM (+41.1%), CD3+ T cells (+17.6%), NK cytotoxicity (+42.4%), and phagocytic index (+44.4%) compared with placebo (all p<0.001). The CD4+/CD8+ ratio remained stable. Adverse events were mild and comparable between groups. IMMUN-5 significantly enhanced both humoral and cellular immune parameters without evidence of immunological dysregulation.

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