Effect of Dentin Pretreatment using Arginine, Polyamidoamine Dendrimer and their Combination on Bond Durability and Micromorphology

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D.O.I.

10.1922/EJPRD_2829Aboelwafa08

• Dalia M. Elasser
• Nesreen Y. Mohammed
• Mona R. Aboelwafa

Abstract

Objectives To analyze the impact of dentin pretreatment using arginine (L.arg), amineterminated polyamidoamine dendrimer (PAMAM-NH2), and their combination on microtensile bond strength (µTBS) and resin-dentin micromorphology. Methods Demineralized middle-coronal dentin surfaces of eighty sound molars were randomly distributed among four groups depending on the implemented surface pretreatment (n=20): (1) 8% L.arg, (2) PAMAM, (3) PAMAM + L.arg, and (4) untreated dentin (control group). Following bonding and composite build-up procedures, each group was subdivided into; the non-aged
subgroup, where the µTBS was measured immediately, and the aged subgroup, where the specimens were thermo-cycled before µTBS. Resin-dentin sticks were prepared for µTBS testing. The failure mode distribution was analyzed. The resin-dentin interface was investigated using Field-emission scanning electron microscopy. A two-way ANOVA was utilized, followed by Tukey’s post hoc test. Results before thermo-cycling, group 1 had the highest µTBS mean value followed by groups 2 and 4 without a significant difference. While group 3 showed the statistically least µTBS (P <0.001). After thermo-cycling, PAMAM pretreatment significantly increased the µTBS Conclusions PAMAM pretreatment possessed a favorable impact on the dentin- resin bonding durability. Clinical relevance PAMAM -NH2 and L.arg surface treatment could improve immediate and long-term stability of the dentin–resin bonding.
Keywords
Microtensile Bond Strength
Polyamidoamine Dendrimer
L. arginine
Resin-Dentin Micromorphology
Adhesion