European Journal of Prosthodontics and Restorative Dentistry

Evaluation of the Antimicrobial Effect on Enterococcus Faecalis of Bioceramic Cements with and without Silver Nanoparticles

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Cover Date

June 2023

Print ISSN

0965-7452

Electronic ISSN

2396-8893

Vol

31

Issue

2

Abstract

Objective: To compare the antimicrobial effect of bioceramic endodontic cements with
and without silver nanoparticles on Enterococcus faecalis. Materials and Methods: Six
groups were evaluated (n=8), and divided according to the materials: MTA Flow + AgNP ;
MTA Flow Group; Bio C Repair + AgNP; Bio C Repair; PBS Cimmo + AgNP; PBS Cimmo. The
groups were maintained for 72 h in the suspension of Enterococcus faecalis in Simulated
Body Fluid (SBF). Samples of 100 µL were removed from the suspension at 0, 24, 48 and
72 h and seeded in triplicate in a Petri dish. Colony forming units (CFU) were counted
using a colony counter. All procedures described were performed in a laminar flow
chamber. Two-way ANOVA followed by Tukey’s post hoc test and paired t-test were used
for statistical analyses (α = 0.05). Results: The addition of silver nanoparticles resulted
in a statistically significant difference for MTA and CIMMO PBS cements (p‹0.05), with
the lowest bacterial growth being shown by the MTA group. For all groups, only the
times of 48 and 72 h presented results without differences. Conclusion: The addition of
silver nanoparticles to bioceramic cements was efficient to promote an acceleration of
bacterial death.

Keywords
Silver Nanoparticle
Endodontics
Bioceramics
Enterococcus faecalis